Sample and Library QC

Sample quality is crucial for successful library preparation and, ultimately, the accuracy of your downstream analysis. To ensure the highest quality of your samples, we provide various methods of quality control to determine their integrity, concentration and length distribution. We employ electrophoresis using the Agilent Tapestation for genomic DNA and PCR products and the Fragment Analyzer for RNA, genomic DNA, and PCR products. For other types of samples, we can perform analysis with user-provided kits. We also offer quantification using either a spectrophotometer (Nanodrop or Spark plate reader) or fluorimeter (Quantus or Spark plate reader). 

For libraries, we use a combination of methods for measurement and quantification, including electrophoresis with the Tapestation or Fragment Analyzer, qPCR with the KAPA library quantification kit, and fluorimetry with the Quantus or plate reader. We recommend using a combination of these methods for the most accurate results.