In Situ Gene Expression
Xenium In Situ
The Xenium In Situ enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.
Xenium measures gene expression in tissue sections derived from either formalin fixed and paraffin embedded (FFPE) or fresh frozen (FF) tissue samples placed on Xenium Slides. The sample area for tissue placement is 10.45 mm x 22.45 mm. The Xenium In Situ Gene Expression workflow can be paired with optional cell segmentation staining. After the tissue section is placed on a slide, the probes from the panel are hybridized and amplified.10x offers a variety of pre-designed human and mouse panels, from multi-tissue and cancer panel to tissue-specific panels. In addition, individual panels can be customized by adding up to 100 additional genes. It is also possible to design and use a custom panel containing up to 480 genes.
After probe amplification, the slides are loaded into Xenium Analyzer. This instrument integrates high-resolution imaging and onboard data analysis enabling the processing of 400 mm² of tissue in <50 hrs (with nuclei-based segmentation).
