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Research Infrastructure National Center for Medical Genomics

 

Documents

Documents

Guidelines:

Sample delivery guidelines

Cell suspension delivery guidelines


Isolation manuals:

RNA isolation using TRI Reagent and ReliaPrep kit


Sample and library QC manuals:

ScreenTape D1000

ScreenTape HSD1000

ScreenTape RNA

ScreenTape gDNA

Fragment Analyzer RNA (15 nt)

Fragment Analyzer HS RNA (15 nt) 

Fragment Analyzer dsDNA  (1-500 bp)

Fragment Analyzer dsDNA (35-5000 bp) 

Fragment Analyzer HS Genomic DNA 50 kb

Fragment Analyzer HS NGS (1-6000 bp)

QuantiFluor dsDNA

QuantiFluor ssDNA

QuantiFluor RNA

KAPA library quantification kit


Library prep manuals:

xGen DNA Library Prep EZ UNI Kit

KAPA Hyper Prep Kit

QuantSeq 3‘ mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices (12nt)

NEBNext Ultra II RNA Library Prep Kit for Illumina

16S library preparation protocol from Illumina

Cell Preparation for Single Cell Protocols

Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index)


Sequencing manuals:

MiSeq System Denaturate and Dilute Libraries Guide

NextSeq System Denaturate and Dilute Libraries Guide

NovaSeq 6000 Denaturate and Dilute Libraries Guide

Element Adept Rapid PCR-Plus Protocol

Element Adept Standard Protocol

Element AVITI System Guide

MGIEasy Universal Library Conversion Kit (App-A)

DNBSEQ-G400RS High-throughput (Rapid) Sequencing Set







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Brno

We are located in the second largest city of the Czech Republic.

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